Making Chlamydomonas Reinhardtii a Better Model Organism: Tackling the Inefficiency of Nuclear Transgene Expression and Improving Methods for the Generation and Characterization of Insertional Mutant Libraries
نویسنده
چکیده
....................................................................................................................ii TABLE OF CONTENTS ..................................................................................................iv ACKNOWLEDGEMENTS .............................................................................................. vii CHAPTER 1: Literature Review .................................................................................... 1 Chlamydomonas reinhardtii: a useful model organism for many purposes .................. 2 Photosynthetic properties of C. reinhardtii ..................................................... 3 Genetic transformation of C. reinhardtii ......................................................... 5 Utilization of the C. reinhardtii chloroplast as a protein production factory ..... 5 Overview of C. reinhardtii properties that restrict its utility as a model organism ......... 7 Utilization of polycistronic genes in eukaryotic organisms ........................................... 9 Viral 2A peptides .......................................................................................... 10 Mutagenesis and gene targeting ................................................................................ 13 Insertional mutagenesis ............................................................................... 16 CHAPTER 2: Development of an improved and versatile viral 2A platform for expression of polycistronic nuclear genes in Chlamydomonas reinhardtii .......... 21 Abstract ...................................................................................................................... 22..................................................................................................................... 22 Introduction ................................................................................................................ 23
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